LIMNOLOGICAL CHEMISTRY / ION CONCENTRATIONS


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LIMNOLOGICAL CHEMISTRY / ION CONCENTRATIONS
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PRINCIPAL INVESTIGATOR: W. Berry Lyons
Address:Byrd Polar Research Center, Ohio State University, 1090 Carmack Rd, Scott Hall, Columbus OH, 43210-1002
Phone:(614) 688-3241
E-Mail:lyons.142@osu.edu

OTHERS: Kathy Welch
Address:Byrd Polar Research Center, Ohio State University, 1090 Carmack Rd, Scott Hall, Columbus OH, 43210-1002
Phone:(614) 688-4593

KEYWORDS: lake, limnology, solute concentrations, inorganic, anions, cations, chemistry

ABSTRACT: As part of the Long Term Ecological Research (LTER) project in the McMurdo Dry Valleys of Antarctica, a systematic aqueous geochemical sampling program has been undertaken. A series of terrestrial water samples have been collected and analyzed for major ion chemistry by ion chromatography. The concentrations of ions cover a wide range of total dissolved solids from fresh to hypersaline lake waters. This dataset shows concentrations of lithium, sodium, potassium, magnesium, calcium, iron, chlorine, bromine, silicon, fluorine, SO4, and dissolved inorganic carbon found in various depths of Taylor Valley lakes.

VARIABLES: limno run, location name, location code, date, depth (m), Li (mg/L), Li (mM), Li comments, Na (mg/L), Na (mM), Na comments, K (mg/L), K (mM), K comments, Mg (mg/L), Mg (mM), Mg comments, Ca (mg/L), Ca (mM), Ca comments, F (mg/L), F (mM), F comments, Cl (mg/L), Cl (mM), Cl comments, Br (mg/L), Br (mM), Br comments, Si (mg/L), Si (mM), Si comments, SO4 (mg/L), SO4 (mM), SO4 comments, Fe (mg/L), Fe comments, DIC (mg C/L), DIC (mM C/L), DIC comments, file name

RESEARCH LOCATION: Study areas were located in the McMurdo Dry Valley of southern Victoria Land, Antarctica (in the western coast of the Ross Sea at 77°S). Specific sites include the eastern portion of Lake Bonney, the western portion of Lake Bonney, Lake Fryxell, Lake Hoare, Lake Joyce, Lake Miers, Lake Garwood, and Lake Vanda. Samples and original data are stored in the Department of Geology, University of Alabama, Tuscaloosa, AL, Bevill Building, Room 213.

METHODS: Lake sampling was carried out by lowering a Niskin bottle through a hole in the ice cover of the lake to specified depths. Samples were then collected in precleaned plastic bottles. Separate aliquots were collected in serum vials for pH and dissolved inorganic carbon (DIC) measurements. Samples were returned to field laboratories for processing and for time-sensitive analyses such as pH.

Dedicated sampling equipment for each lake has been important for maintaining the integrity of the samples. For example, the range of Cl- concentration in the investigated lakes varies by more than three orders of magnitude. This carryover between samples becomes a potential problem. Carryover can also be a potential problem during analysis, and therefore analytical blanks are run to monitor this.

The initial sample processing was carried out in field laboratories. A 100-ml sample aliquot was filtered for each anion and cation sample. All samples were filtered through 0.4-µm Nucleopore filters, usually within 12h of collection. The cation samples were filtered into acid-washed polyethylene bottles which had been rinsed with DI water, while the anion samples were filtered into DI-washed bottles. The cation samples were acidified by adding 0.5% (v/v) of concentrated HCl. Quality control was maintained by carefully rinsing all filtration apparatus with DI between samples and by processing filtration blanks which were later run as samples.

The majority of water chemistry samples were returned to the Crary Laboratory, McMurdo Station, Antarctica, for analysis. Some of the samples were returned to the laboratory at the University of Alabama for analysis owing to time constraints during the field season.

DX-300 ion chromatographic system was used for the major ion analyses. The system included a gradient pump module, high-pressure injection valve with a 25-µm sample loop, a Dionex conductivity detector (CDM-3) advanced computer interface and automated sampler. The timed events and data collection were controlled by the Dionex AI-450 chromatography software for Windows. The same ion chromatographic system was used but was switched back and forth between anion and cation configurations.

For the anions, a Dionex Ionpac AS4A-SC analytical column (250x4mm I.D.) and AG4A-SC guard column (50x4mm I.D.) were used along with an Anion Self-Regenerating Suppressor-1. The eluent was 1.8 mM Na2CO3-1.7 mM NaHCO3. The gradient pump flow-rate was 2 ml/min and the background conductivity was ca. 16 µS.

For the cations, the Dionex Ionpac CS12 analytical column (250x4mm I.D.) and CG12 guard column (50x4mm I.D) were used with a Cation Self-Regenerating Suppressor-1. The eluent was 0.020 M methanesulfonic acid. The eluent flow-rate was 1.0 ml/min and the background conductivity was ca. 200-250 nS.

The stock standard solutions used for a typical batch of samples from the lakes are specified below:

 
CATION		CONCENTRATION (mg/L)	ANION		CONCENTRATION (mg/L)
  Na+     	   	100		  Cl-			100
  K+		 	 20		SO4(2-)			100
  Mg(2+)	 	 	 50		   F-			 10
  Ca(2+)	 	 	 50		  Br-			  2
  Li+           	  	  1	

Appropriate dilutions of the stock standards were used to prepare a range of standards for calibration. Owing to the high salt concentrations in many of the samples, dilutions were made before the samples were run. Dilutions ranged from 1:2 for Lake Hoare surface water up to 1:6000 for the Lake Bonney deep water. The samples were diluted by serial dilution, using plastic microbeakers and adjustable pipettors.

Replicate and duplicate samples were run daily. Usually, each sample was injected twice and samples from each batch were run in duplicates to check the precision of the dilutions. In almost every case, the relative standard deviation of the duplicates was less than 1%, even with dilutions of 1:6000. In addition, analytical blanks and filtration blanks were analyzed to check the quality control.

Lake water samples were analyzed in the field laboratories for DIC with an infrared gas analyzer. The DIC data was used with the other major ion data to calculate ionic balances as a further check on the data quality. Results of the ionic balance calcultaions are summarized below:

 
DIFFERENCES BETWEEN TOTAL ANIONS AND TOTAL CATIONS (%), WITH STANDARD DEVIATIONS IN PARENTHESES:
  Lake Hoare		2.1 (2.1)
  Lake Fryxell		3.0 (2.0)
  East Lake Bonney	1.8 (1.4)
  West Lake Bonney	1.4 (1.0)

TIMING: Samples were collected from the following sites and dates:

 
Season		East Bonney	West Bonney	Hoare		Fryxell						
91-92								21-Nov-91
 								25-Dec-91
 								22-Jan-92

93-94		27-Oct-93	29-Oct-93	5-Nov-93	16-Nov-93
 		24-Nov-93	26-Nov-93	1-Dec-93	7-Dec-93
 		21-Dec-93	23-Dec-93	30-Dec-93	7-Jan-94
 						19-Jan-94	18-Jan-94
 						
94-95		2-Nov-94	5-Nov-94	10-Nov-94	14-Nov-94	
 		23-Nov-94	25-Nov-94	30-Nov-94	4-Dec-94
 		17-Dec-94	19-Dec-94	24-Dec-94	30-Dec-94
 		10-Jan-95	12-Jan-95	20-Jan-95	17-Jan-95
 		
95-96		19-Sep-95	21-Sep-95	7-Sep-95	14-Sep-95
 		6-Oct-95	4-Oct-95	25-Sep-95	28-Sep-95
 		17-Oct-95	19-Oct-95	10-Oct-95	14-Oct-95
 		2-Dec-95	5-Dec-95	21-Nov-95	26-Nov-95
 		1-Jan-96	4-Jan-96	23-Dec-95	27-Dec-95
 		
96-97		3-Nov-96	1-Nov-96	8-Nov-96	26-Oct-96	
 		24-Nov-96	27-Nov-96	2-Dec-96	19-Nov-96
 		15-Jan-97	17-Jan-97	12-Jan-97	9-Jan-97
 		
97-98		8-Nov-97	11-Nov-97	15-Nov-97	18-Nov-97	
 		20-Dec-97	22-Dec-97	26-Dec-97	29-Dec-97
 
98-99	 	9-Nov-1998	11-Nov-1998	 3-Nov-1998	28-Oct-1998
		 6-Dec-1998	 9-Dec-1998 	 1-Dec-1998	23-Nov-1998
		30-Dec-1998	 1-Jan-1999	26-Dec-1998	20-Dec-1998

99-00	 	6-Nov-1999	11-Nov-1999	17-Nov-1999	30-Oct-1999
		 3-Dec-1999	 5-Dec-1999	12-Dec-1999	27-Nov-1999
		28-Dec-1999	30-Dec-1999	 4-Jan-2000	23-Dec-1999



Season		Joyce		Miers		Garwood		Vanda		Vida		Chad		Chad at Narrows
91-92

92-93 												22-Jan-94	3-Jan-94

93-94								17-Jan-94	17-Jan-94

94-95		30-Nov-94	25-Jan-95	24-Jan-95	1-Dec-94

96-97		5-Dec-96	10-Dec-96

97-98		9-Dec-97	5-Dec-97



Season		Don Juan Pond	Dirty Little 	Pony Lake on 	Skua Pond on 	Trough	
				Hoare Pond	Ross Island	Ross Island
93-94		1-Jan-94			27-Jan-94	27-Jan-94

95-96				8-Jan-96

96-97						22-Jan-97

98-99										23-Dec-98



CITATIONS: Welch, K.A., Lyons, W.B, Graham, E., Neumann, K., Thomas, J.M., and D. Mikesell. 1996. Determination of major element chemistry in terrestrial waters from Antarctica by ion chromatography. Journal of Chromatography A 739, 257-263.

COMMENTS:

STATUS: Public Access (Type 1).

VARIABLE DESCRIPTION:

 
VARIABLE	TYPE	DESCRIPTION			UNITS		MISSING VALUE INDICATOR   MINIMUM	MAXIMUM	    PRECISION

Limno run	Text	ID# associated with location,	None		  Required entry	   n/a		  n/a	       n/a
			collection date
Location name	Text	Lake where data was gathered	None		  Required entry	   n/a		  n/a	       n/a
Location code	Text	Abbreviation used for that	None	  	  Required entry	   n/a		  n/a	       n/a
			location
Date		Date	Date sample was collected	mm/dd/yy	  Required entry	   n/a		  n/a	       n/a
Li (mg/L)	Number	Lithium	concentration		milligrams/liter	Null		    0		   50	      0.0001
Li (mM)		Number	Lithium	concentration		millimoles/liter	Null		    0		    5	      0.0001
Li comments	Text	Comments pertaining to		None			Null               n/a            n/a          n/a
			lithium measurement
Na (mg/L)	Number	Sodium	concentration		milligrams/liter	Null		    0		100,000		1
Na (mM)		Number	Sodium	concentration		millimoles/liter	Null		    0		  5,000	       0.01
Na comments	Text	Comments pertaining to		None			Null               n/a            n/a          n/a
			sodium measurement
K (mg/L)	Number	Potassium concentration		milligrams/liter	Null
K (mM)		Number	Potassium concentration		millimoles/liter	Null
K comments	Text	Comments pertaining to		None			Null               n/a            n/a          n/a
			potassium measurement
Mg (mg/L)	Number	Magnesium concentration		milligrams/liter	Null
Mg (mM)		Number	Magnesium concentration		millimoles/liter	Null
Mg comments	Text	Comments pertaining to		None			Null               n/a            n/a          n/a
			magnesium measurement
Ca (mg/L)	Number	Calcium	concentration		milligrams/liter	Null
Ca (mM)		Number	Calcium	concentration		millimoles/liter	Null
Ca comments	Text	Comments pertaining to		None			Null               n/a            n/a          n/a
			calcium measurement
F (mg/L)	Number	Fluorine concentration		milligrams/liter	Null
F (mM)		Number	Fluorine concentration		millimoles/liter	Null
F comments	Text	Comments pertaining to		None			Null               n/a            n/a          n/a
			fluorine measurement
Cl (mg/L)	Number	Chlorine concentration		milligrams/liter	Null
Cl (mM)		Number	Chlorine concentration		millimoles/liter	Null
Cl comments	Text	Comments pertaining to		None			Null               n/a            n/a          n/a
			chlorine measurement
Br (mg/L)	Number	Bromine	concentration		milligrams/liter	Null
Br (mM)		Number	Bromine	concentration		millimoles/liter	Null
Br comments	Text	Comments pertaining to		None			Null               n/a            n/a          n/a
			bromine measurement
Si (mg/L)	Number	Silicon	concentration		milligrams/liter	Null
Si (mM)		Number	Silicon	concentration		millimoles/liter	Null
Si comments	Text	Comments pertaining to		None			Null               n/a            n/a          n/a
			silicon measurement
SO4 (mg/L)	Number	SO4 concentration		milligrams/liter	Null
SO4 (mM)	Number	SO4 concentration		millimoles/liter	Null
SO4 comments	Text	Comments pertaining to		None			Null               n/a            n/a          n/a
			SO4 measurement
Fe (mg/L)	Number	Iron concentration		milligrams/liter	Null
Fe comments	Text	Comments pertaining to		None			Null               n/a            n/a          n/a
			iron measurement
DIC (mg C/L)	Number	Dissolved inorganic		milligrams of carbon	Null
			carbon concentration		per liter
DIC (mM C/L)	Number	Dissolved inorganic		millimoles of carbon	Null
			carbon concentration		per liter
DIC comments	Text	Comments pertaining to		None			Null               n/a            n/a          n/a
			dissolved inorganic
			carbon measurement
File name	Text	Raw data file containing        None                    Null               n/a            n/a          n/a                                                               
                        information

LOG: The original iterations of the 'limnchem' file (limnological chemistry data) were created by Kathy Welch, and submitted to the Institute of Arctic and Alpine Research (INSTAAR) in a July 10, 1997 email message. These files are currently located in the /data1/data/lakes/lakechem directory and contain the following names:

Following discussions between Kathy Welch (who submitted data) and Denise Steigerwald (data manager), it was decided to remove some fields (eg., dilution factor, sample name), and add others (eg. collection site, collection date, comments for each type of measurement) to these files in order to make them more consistent and relational for future references. The resulting files contain the same name, but have 'xls' extensions. (They were altered using Microsoft Excel). Following these revisions, they were imported into Microsoft Access and saved in a table named "Limnological Chemistry 1991-95 (Welch / Lyons)" Because location name, collection date, and depth are all variables for this table, it was possible to combine each of these files in one table. This table was exported as an ascii, text, comma delimited file named "limnchem.dat" and saved in the appropriate directory to make it accessible on the web page. Data for 1998-2000 was submitted to the Data Manager (Kevin Wheeler) as files Limno_IC_9899.xls and Limno_IC_9900.xls. All original files are archived in /data1/data/lakes/lakechem directory.

NOTE: Data contained in these files has been subjected to quality control standards imposed by the investigator. The user of this data should be aware that, while efforts have been taken to ensure that these data are of the highest quality, there is no guarantee of perfection for the data contained herein and the possibility of errors exists. If you encounter questionable data, please contact the MCM LTER data manager (; (303)492-4639) so that the data can be corrected or qualified. Thus, these data may be modified and future data will be appended.